User:Kristina M Holton/Ovarian Notebook 2007 7 16

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Bioanalyzer for Ambion & Qiagen FFPE Kits

First chip did not run -> voltage problem

  • Test plates OK
  • V=IR; V=Q/(eA/d) (V=Q/C) so...Q & I are fine, problem with loading gel (dialectric)
  • Chip pressurizing syringe changed
  • Small RNA assay very temperamental according to Agilent
 Make sure: no bubbles, liquid at bottom of wells
 Suggestion: practice loading technique on sample known to contain purified small RNAs; practice with another chip
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