User:Kristina M Holton/Ovarian Notebook 2007 9 25

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Total RNA Extraction Protocol: SW480 & SW620 cell lines Day 2

BioAnalyzer: NanoChip on cell line samples

Qiagen RNeasy FFPE kit: 12 samples

Notes on Protocol:

  • Step 6: Samples 9,11,12 not well pelleted; 3 is red; 9 is brown; 11 small pellet
  • Step 9: Sample 12 very poorly pelleted
  • Step 14: Sample 7; Sample 10 poorly digested
Sample
Sample ID
ng/ul
260/280
260/230
Vol
Total
Dilution
Total to Illumina
1
502-35932-D
278.11
1.94
1.69
30ul
8.3ug
15ul + 26.7ul H20
4.15ug
2
502-35973-G
191.71
2.0
1.77
30ul5.8ug
15ul + 13.7ul H20
2.9ug
3
502-36090-J
150.43
1.99
1.84
30ul4.5ug
15ul + 7.5ul H20
2.25ug
4
502-36200-M
1708.2
2.02
2.08
30ul51ug
15ul + 241ul H20
25.5ug
5
502-36433-AA
1261.3
1.97
1.71
30ul38ug
15ul + 174ul H20
19ug
6
502-36752-E
732.33
1.93
1.92
30ul22ug
15ul + 95ul H20
11ug
7
502-36839-I
344.33
2.02
1.95
30ul10ug
15ul + 37ul H20
5ug
8
502-36878-B
1612.9
2.02
2.10
30ul48ug
15ul + 226ul H20
24ug
9
502-36925-B
529.03
1.97
2.04
30ul16ug
15ul + 64ul H20
8ug
10
502-37203-AE
120.2
1.94
1.97
30ul3.6ug
15ul + 3ul H20
1.8ug
11
599-35521-E
270.36
1.80
1.19
30ul8.1ug
15ul + 26ul H20
4.05ug
12
599-35521-N
939.11
2.0
1.78
30ul28ug
15ul + 126ul H20
14ug
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