User:Lindenb/Notebook/UMR915/20110103

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20101215        Top        20110104       


Bonne annĂ©e !

VCFAnnotator

wrote a tool to annotate VCf files. see my blog: http://plindenbaum.blogspot.com/2011/01/my-tool-to-annotate-vcf-files.html Running this tool with belgium data.

Comparing mask/no mask

using ftp://ftp-trace.ncbi.nih.gov/1000genomes/ftp/pilot_data/data/NA20772/sequence_read/ERR004053_*.recal.fastq.gz

mysql -D hg18 -u anonymous -e 'select distinct chrom,txStart,txEnd from knownGene where chrom="chr1" ' -N > /tmp/jeter.txt
java -jar dist/maskfasta.jar -r /tmp/jeter.txt -e 10000 /GENOTYPAGE/data/users/lindenb/MASKBWA/NO_MASK/chr1.fa >  /GENOTYPAGE/data/users/lindenb/MASKBWA/WITH_MASK/chr1.fa
[lindenb@srv-clc-02 NO_MASK]$ sed 's/N/\nN\n/g' NO_MASK/chr1.fa | grep N | wc -l
22250000
[lindenb@srv-clc-02 NO_MASK]$ sed 's/N/\nN\n/g' WITH_MASK/chr1.fa | grep N | wc -l
108399153

for each chr1.fa:

 /usr/local/package/bwa-0.5.9rc1/bwa  index  chr1.fa
 /usr/local/package/bwa-0.5.9rc1/bwa aln chr1.fa ../FASTQ/ERR004053_1.recal.fastq.gz > aln1.sai
 /usr/local/package/bwa-0.5.9rc1/bwa aln chr1.fa ../FASTQ/ERR004053_2.recal.fastq.gz > aln2.sai
 /usr/local/package/bwa-0.5.9rc1/bwa sampe chr1.fa aln1.sai aln2.sai ../FASTQ/ERR004053_1.recal.fastq.gz ../FASTQ/ERR004053_2.recal.fastq.gz > file.sam
 /usr/local/package/samtools-0.1.10/samtools faidx chr1.fa
 /usr/local/package/samtools-0.1.10/samtools view -b -t chr1.fa.fai file.sam > file.bam
 /usr/local/package/samtools-0.1.10/samtools sort file.bam sorted
 /usr/local/package/samtools-0.1.10/samtools index sorted.bam
 /usr/local/package/samtools-0.1.10/samtools pileup -vcf chr1.fa sorted.bam | awk '($3=="*"&&$6>=50)||($3!="*"&&$6>=20)' | cut -d '      ' -f 1-4 | sort | uniq > pileup.txt


[lindenb@srv-clc-02 WITH_MASK]$ wc -l pileup.txt 
24921 pileup.txt
[lindenb@srv-clc-02 NO_MASK]$  wc -l pileup.txt
26062 pileup.txt
[lindenb@srv-clc-02 MASKBWA]$ comm -12 WITH_MASK/pileup.txt NO_MASK/pileup.txt | wc
  13573   54292  266327
[lindenb@srv-clc-02 MASKBWA]$ comm -13 WITH_MASK/pileup.txt NO_MASK/pileup.txt | wc
  12489   49956  249431
[lindenb@srv-clc-02 MASKBWA]$ comm -23 WITH_MASK/pileup.txt NO_MASK/pileup.txt | wc
  11348   45392  229152

on position appears masked but not w/o mask:

 chr1	100005960	c	A

in Masked

 /usr/local/package/samtools-0.1.10/samtools tview WITH_MASK/sorted.bam WITH_MASK/chr1.fa
  100005921 100005931 100005941  100005951 100005961 100005971
tgctaattggtcagattggagatggaatca*tggggggtcgacgtgaggttttcttgctgtcttct
....G.........G............... MM.......R.A....RK.................
..              ,,,,,,,,,,,,,,cac,,,,,,,,,a,,,,a,,,  ,,,,,,,,,,,,,
....                ..........*CA.......A.A.......N.......  ,,,,,,
..                  ..........*CA.......A.A...............    ,,,,
G...G..G..          ..........*CA.......A.A...............
....G.........G..T..                 ...........T.................
....G.......                         .....A.....T.................
,,,,g,,,,,,,,,g,,,,,,,,,,,,,,,*,,,,,       ..............G........

NO MASK:

/usr/local/package/samtools-0.1.10/samtools tview NO_MASK/sorted.bam NO_MASK/chr1.fa
  100005931 100005941 100005951 100005961 100005971 100005981
tcagattggagatggaatcatggggggtcgacgtgaggttttcttgctgtcttctgttcctgggtg
          ..........CA.......R.A.....K............................
          ..........CA.......A.A.......N.......
                          ...........T.........................
                          .....A.....T.........................
                              .A..................................
                                ..............G...................
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