User:Linh N Le/Notebook/2009/08/27

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

To Do

  • We are running low on tips and tubes, so I need to autoclave some today and tomorrow before I leave


  • Doing the rest of the yellow tips in the red boxes
    • about 8 in total
  • Also showing Brian how to use the Autoclave

Things that I dont want to fall into obscurity

  • Koch mentioned that I should make sure any work I have done does not get forgotten
  • I'm pretty sure I have documented all the work I've done
  • Here's a list of things that are useful to the lab that I may not want to die:
    1. Inventory Stuff
    2. Profiling laser beams? (it is a bit of an archaic method, but it works on a budget)
  • The laser stuff is all on the private wiki, might be a good idea to have it transferred to the public
  • My Comic (on the private wiki). Anyone is allowed to put new comics in, they dont even have to follow the "stick figure" approach is use.
    • I have a few ideas that I may need to draw in retroactively, i've just been too busy/lazy to do them lately



  • Andy, Brigette and I resuspended some Rhodamine Tubulin today (made 4 aliquots) 1ul in ea aliquot (5mg/ml)
  • We then took 1 aliquot of RT and then added it to an aliquot of unlabeled tubulin
    • We have 6, 1ul aliquots that are 15% RT
  • I went ahead and polymerized one of the mixed aliquots and we created motility soln
  • Andy tried a motility assay, but it failed
    • Brigette and I forgot to add Dextrose to the motility soln and the MT's became "kinked"
  • Retrying with dextrose now added
  • Note: The 15% RT is enough to see, which is good
  • Success! We got to see motility and the MT's are really long and easy to see
  • They are moving quite fast and the AF is working great
  • No camera (in tweezers lab) so no photo/video footage. Hopefully it works again tomorrow and Andy can bring over the cam
  • Andy Maloney 23:22, 27 August 2009 (EDT): I'm glad to hear it worked. Did you guys change out the ATP to get it to work or did you use the same stuff?
  • Steve Koch 23:48, 27 August 2009 (EDT): Awesome! Glad to hear about motility. I think we'll have a camera soon?
  • Linh N Le 02:30, 28 August 2009 (EDT) We added the dextrose and that fixed all the problems (brigette did most of the work, her notebook will state if she did anything else differently)
Personal tools