User:Lu Wang/Notebook/Team Allergy/2010/06/30
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Yesterday, we plated the allergen panel. If we have transformants for hpRNA today, then we will culture colonies and miniprep them for their DNA. Arabadopsis gDNA also arrived, so we will attempt to PCR out introns PAL and PME. We will also redo ligations for amiRNA, including a negative control.
Culture Colonies Only Bet S worked, but there are only two colonies, so they may be background. We cultured a colony for each tube at 10am.
Miniprep The concentrations of plasmids after the miniprep are 80ng/μL and 108ng/μL
PCR for PAL and PME