User:Mackenzie K. Kelley/Notebook/Experimental Biochemistry/2017/09/13

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Objective Dialysis on a AuNP solution to determine: how big the AuNP complexes are how strongly the stabilizer is bound to the AuNP if we can change the stabilizer how quickly the molecules migrate through the tubing Remake AA solutions (didn't work last time) Description Stabilizer exchange prepared and ran dialysis on two dialysis tubes: 1 with DI water and 25 mM HdMe2ImCl, 1 with AuNP solution and 25 mM HdMe2ImCl (Cysteine in BSA 90 ratio Au:AA) Ever 30 minutes, remove 1 mL of solution from jar and measure the absorbance at 211 nm using the spectrophotometer note: we were taking the solution out of the dialysis bag itself Other exchanges Using same procedure to prepare dialysis tubes, prepared a total of 4 dialysis tubes. Dialysis 3 mL AuNP stabilized with basic AA against Glycine buffer Dialysis 3 mL AuNP stabilized with basic AA against water Dialysis 3 mL AuNP stabilized with BSA against Glycine buffer Dialysis 3 mL AuNP stabilized with BSA against water Data