September 3, 2013
Execute procedure for
making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.
Stock Solutions and Dilutions
Adenosine MW: 267.24g/mol
Inosine MW: 268.2g/mol
Original stock solutions:
Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M
Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M
Dilutions to make, each with total volumes of 1mL:
|Adenosine solution concentrations (M)
||Volume stock solution added (μL)
||Inosine solution concentrations (M)
||Volume of stock solution added (μL)
All peaks at 259nm.
Calculations and Analysis
Determine the standard deviation for your data points.
Determine the confidence interval for 90% and 95% confidence.
Determine if any data can be ruled out using a Q-test.
- Microliter amounts were measured with a micropipet
- 50mL volumetric flasks were used to make stock solutions of both adenosine and inosine