User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/17

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September 17, 2013


Execute the following procedure from Dr. Hartings' notebook, in order to determine the amount of potassium ferricyanide/sodium dithionite that is required to completely oxidize/reduce (respectively) horseradish peroxidase.


Oxidation with Potassium ferricyanide

Reduction with Sodium dithionate


Results for both the oxidation and reduction were not observed as expected. This may be because the horseradish peroxidase oxidized/reduced by the first measurement, and subsequent measurements showed no change in absorbance because of this.
Once in each trial, the solution was spiked with more concentrated reagent, which showed very little change as well, validating the above conclusion.


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