User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/17

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September 17, 2013

Objective

Execute the following procedure from Dr. Hartings' notebook, in order to determine the amount of potassium ferricyanide/sodium dithionite that is required to completely oxidize/reduce (respectively) horseradish peroxidase.

Data

Oxidation with Potassium ferricyanide
Image:2013_0917_HRP_oxidation_spectra.PNG

Reduction with Sodium dithionate
Image:2013_0917_HRP_reduction_spectra.PNG

Notes

Results for both the oxidation and reduction were not observed as expected. This may be because the horseradish peroxidase oxidized/reduced by the first measurement, and subsequent measurements showed no change in absorbance because of this.
Once in each trial, the solution was spiked with more concentrated reagent, which showed very little change as well, validating the above conclusion.


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