User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/05

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Experimental Biological Chemistry: Fall 2013 Main project page
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November 5, 2013

Objective

  1. Atomic Absorption of nanoparticles
  2. UV-vis measurements of Lysozyme and Myoglobin nanoparticles

Description

Atomic Absorption:

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner

Data

Atomic Absorption
Image:2013_1105_gold_standards_corrected.PNG
Image:2013_1105_myoglobin_nanoparticles_corrected.PNG
Image:2013_1105_lysozyme_nanoparticles_UVvis_corrected.PNG Image:2013_1105_HRP_nanoparticles_corrected.PNG

UV-vis Absoprtion
Image:2013_1105_lysozyme_nanoparticles_UVvis.PNG
Image:2013_1105_myoglobin_nanoparticles_UVvis.PNG

Notes


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