User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/06

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Experimental Biological Chemistry: Fall 2013 Main project page
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November 6, 2013

Objective

  • Make Lysozyme and Hemoglobin nanoparticles

Description

Synthesizing Hemoglobin and Lysozyme Nanoparticles

  • Hemoglobin
  1. 30
  2. 50
  3. 70
  4. 90
  5. 110
  6. 130
  7. 150
  8. 170
  9. 190
  10. 210
  • Lysozyme
  1. 5
  2. 10
  3. 15
  4. 20
  5. 25
  6. 30
  7. 35
  8. 40
  9. 45
  10. 50
  • Make stock solutions
  • Make samples in small test tubes and cap with aluminum foil
  • Heat samples at 80C for 4 hours


Image:2013_1106_nanoparticle_info.PNG

Notes

  • Next time: samples will be centrifuged, 1mL will be taken off for UV-vis measurements, remaining 4mL will be used for AA measurements





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