User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/26

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Ag:BSA Nanofibers & Preparing Au:Myoglobin Nanofibers for Cell Culture Main project page
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February 26, 2015

Objectives

  • Modify Ag:BSA nanofiber synthesis procedure
  • Prepare Au:Myoglobin nanofiber-coated scaffolds for cell culture

Ag:BSA Nanofibers: Results

  • Set 1 (All ratios, room temperature)


  • Set 2 (All ratios, heated to 80C)


  • Set 3 (All ratios plus 1uL Au, heated to 80C)


Ag:BSA Nanofibers: Sample Prep

Image:2014_0226_Ag_BSA_ratios.PNG

Au:Myoglobin Nanofibers: Sample Prep for Cell Culture

Image:2014_0211_myo_bsa_NPs.PNG

  • Solutions:
    • Au: 0.011g in 10mL to make 2.793mM solution
    • Myoglobin: 0.011g in 10mL to make 0.0621mM solution
    • TRIS dopamine: 0.038g dopamine hydrochloride and 0.024g TRIS in 20mL to make 10mM solution at pH 8.46

Plain Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Three test tubes filled with scaffolds and water
  • Autoclaved

Brute Force Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above method
  • Nanofibers dried onto PLA scaffolds
  • Scaffolds submerged in water
  • Autoclaved

Original Method for Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution
  • Autoclaved

Polydopamine-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Scaffolds submerged in water
  • Autoclaved

Polydopamine Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution
  • Autoclaved



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