User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/19

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February 19, 2013 Re-hybridize: 2.5μM 4 Strand DNA with 3.5μM ThT 2.0μM 4 Strand DNA with 3.5μM ThT 2.5μM 8 Strand DNA with 3.5μM ThT Solutions were heated at approximately 70°C for 25 minutes Hybridize:DNA and ZnPPIX solutions using same method as ThT example: (265.6μM 8 Strand DNA)(v)=(3.5μM 8 Strand DNA)(250μL)<br.> v=3.29μL of 265.6μM 8 Strand DNA in 246.71μL TRIS buffer solution <br.> combined with 250μL 5μM ZnPPIX, solution is heated for hybridization 3.5μM 4 Strand DNA with 5μM ZnPPIX 3.0μM 4 Strand DNA with 5μM ZnPPIX 2.5μM 4 Strand DNA with 5μM ZnPPIX 2.0μM 4 Strand DNA with 5μM ZnPPIX 1.5μM 4 Strand DNA with 5μM ZnPPIX 3.5μM 8 Strand DNA with 5μM ZnPPIX 3.0μM 8 Strand DNA with 5μM ZnPPIX 2.5μM 8 Strand DNA with 5μM ZnPPIX 2.0μM 8 Strand DNA with 5μM ZnPPIX 1.5μM 8 Strand DNA with 5μM ZnPPIX Solutions were heated at approximately 70°C for 25 minutes