User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26

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March 26, 2013 Dilutions of AuNP, MB, and thiol DNA Solution Goal: dilute solution to 1-0.5nM AuNP<br.> (9.7nM)V=(4.85nM)(100μL)<br.> V= 50μL 9.7nM AuNP in 50μL<br.> Serial Dilutions with 50mM HEPES buffer: 4.85nM<br.> 2.425nM<br.> 1.2125nM<br.> 0.61nM<br.> 0.303nM<br.> FCS Data 0.3nM Τ=0.00018 ms<br.> N= 0.41 0.61nM Τ=184.456 ms<br.> N= 620 0.3nM (trial 2) Τ=0.2046 ms<br.> N= 12 o.61nM (trial 2) Τ=0<br.> N=0 Prepare Smaller Intervals of Diluted Samples: (2.425nM)V=(0.5nM)(50μL)<br.> V=10.3μL in 39.7μL HEPES buffer<br.> 0.4 nM → 8.2μL in 41.75μL<br.> 0.3 nM → 6.2μL in 43.8μL<br.> 0.2 nM → 4.1μL in 45.95μL<br.>