User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/06

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June 6, 2013

Zinc Protopophoryin Measurements Continued

Fluorescence data from yesterday 06/05/2013, and today. Image:Fluor_data_thiol_DNA,_AuNP,_ZnPPIX_0605_and_0606.png


Samples excited at approximately 420nm. We ran a new ZnPPIX/DNA sample again and it still showed no fluoresence. We tried heating the sample (at 75°C for about 30 minutes) as well, and still didn't see any. A ZnPPIX/AuNP sample was made to ensure the fluorescence was not due to the AuNPs, and though there appears to be a small amount of signal no peaks were observed.
We complied relevant ThT, MB, and ZnPPIX data and observations from the last few weeks.
Preparation work for the AuNP silica-coating reaction was completed.
Research completed to determine adequate buffer to use in AuNP@SiO2 crosslinking reaction.

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