Entry title
Ran a trial in OceanView for Absorbance readings.
Run at 70°C set through Qpod
- Run A Wizard
- Select spectroscopy
- JAZ is the correct hardware device. Click next
- Select absorbance and click next
- Select integration time as 1 ms
- Select scans to average 10
- Click next
- Open lamp. Click lightbulb to store reference spectrum. Click next
- Close lamp. Click the lightbulb to store dark spectrum. Click finish
- Open lamp. Click save graph to files. Configure
- Save the first available scan every 1 minute
- Stop after 2 hours
- Save to directory in a dropbox folder - 02-20-H2O-70
- File format- Tab separated with headers
- Create file name - mb_opd_acetate_h202_h20_70_
- Click apply
- When ready to run, click play button, then click the save graph to files button a second time.
Cuvette at start time contains-
- 0.675 mL (2.8 mg OPD in 2.8 mL H2O)
- 0.75 mL dilute H2O2 (0.1135 μL H2O2 and 0.8865 μL H2O)
First reading taken after 45 seconds.
0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Acetate buffer in 1 mL H2O)
Steps were repeated at same temperature with 1 mg Mb/KCl/Citrate buffer in 1 mL H2O
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