User:Maria Briscione/Notebook/Chem 571/2011/10/04
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Determine a concentration of MBP that yields an accurate UV-Vis Spectrum.
Estimated concentrations of MBP at 0.2, 2.0, 10.0, 100.0, and 1000.0 µg/mL were analyzed in the UV-Vis. Based on the resulting spectrum the estimated 100 µg/mL dilution was used to determine the molar absorptivity. The accurate concentration of the estimated 100 µg/mL solution was initially determined. The known value of the true concentration of the "2 g/mL" solution was determined to be 2.66055 µg/mL by using the equation of the line of the protein + Bradford Reagent Standards ( y = 0.218x). The absorbance of the protein ("2 µg/mL") in water was the y value ( 0.116) and an x value (concentration) was determined to be 2.66055 µg/mL.
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