User:Maria Briscione/Notebook/Chem 571/2011/11/01

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Objective

The purpose of the present experiments were to synthesize gold nanoparticles and to run PCR

Description

In order to synthesize gold nanoparticles stock solutions of chloroauric acid (2.9 mM) and BSA (15.5 µM) were created. Previously made BSA stock (15 µM) was also used. The effect of acid, in the form of HCl on nanoparticle formation was examined. The effects of time were also determined. Four test tubes containing the following solutions were created.

  1. 1 mL choloroauric acid (.29 mM) + 1 mL BSA (1.5 µM) + 8 ml distilled water
  2. 1 mL choloroauric acid (.29 mM) + 1 mL BSA (1.5 µM) + 8 ml distilled water + 3 M HCl
  3. 1 mL choloroauric acid (.29 mM) + 1 mL BSA (1.55 µM) + 8 ml distilled water
  4. 1 mL choloroauric acid (.29 mM) + 1 mL BSA (1.55 µM) + 8 ml distilled water + 3 M HCl

The reactions were allowed to proceed in an 80-degree C oven for 50 min followed 10 min at room temperature for a total of 3 hours. The reactions were covered in aluminum foil and allowed to sit over night.

note the the order of which the solutions were added is as follows: water, BSA, chloroauric acid, and HCl (for tube 2 and 4)

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PCR was conducted following the procedure outlined on Sept 20:

Forward and reverse PCR primers were determined in accordance to the guidelines set forth by the Quick Change Manual.

Then a PCR reaction was carried out using previously prepared primers.

  1. 43 μL of ddH2O was added
  2. 1 μL on a 0.5 μ/μL of template DNA
  3. 1 μL of a 10mM dNTP solution
  4. 1.25 μL of a 100 ng/μL solution of forward and reverse primers
  5. 1 μL of enzymes were added to the solution
  6. 25 μL of wax was added slowly to the surface of the solution
  7. added to thermocycler

Data

Grey boluses were seen, however no purple fibers were observed. Older chloroauric acid was used, so this could possibly be the cause of the lack of product. HCl tubes had nothing.

Notes

This area is for any observations or conclusions that you would like to note.


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