User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/02

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Transformation failed :(


  • As my transformed strains didn't grow, I needed to repeat the experiment (both ligation and transformation. I think that things went wrong during the ligation 22°C-10 min step, as I set a soft agitation when it was not required).


  • I repeated the ligation using restricted plasmid PSB1T3 restricted with ECO RI and PST I as a control, but I just added ligase. The ligation of luciferase and the double terminator was stored in the fridge with the name "ligación luc TT Mar".



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