User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/10/12

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Assay for a functional luciferase using a Moonlight luminometer


  • Today in the morning, I measured if there was any emission of light in the following cultures, guided by the kind help of Chris Wood Phd., and the willingness of Susana López, Phd. to lend her luminometer. The measurements are as follows:



Measure Control Medium Bacteria ClickBeetleGreen ClickBeetleRed PPlucwt PPmluc PPmlucJ23102
1 85 151 317 2181 76 143 231 241
2 60 116 125 4144 72 91 102 107
3 72 97 81 7748 58 76 92 79
luci 1 87 131 111 22443367 79 85 128 109
luci 2 84 109 93 17592397 74 69 91 77
luci 3 68 89 94 17037342 70 74 78 82



  • The Control sample implies a tube containing citratum sodium medium prepared as described here.


  • The Medium sample contains LB as a negative control.


  • The Bacteria sample is DH5-α without transforming plasmids (which served as a bacterial negative control); the rest of the cultures were transformed with the corresponding plasmid:
  1. Click Beetle Green Luciferase
  2. Click Beetle Red Luciferase
  3. Photinus pyralis wild type luciferase
  4. Photinus pyralis mutated luciferase under J23102 promoter





  • The second graph excludes the functional click beetle green luciferase. Colors for each sample are:
  1. Control -> Purple
  2. Medium -> Blue
  3. Bacteria -> Pink
  4. ClickBeetleRed -> Black
  5. PPlucwt -> Green
  6. PPmluc -> Orange
  7. PPmlucJ23102 -> MISSING!!!




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