User:Mark Skaggs/Notebook/Lab Four: Plantae and Fungi
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Objective: To observe and understanding the differences of plant structures and the function of fungi
Methods: 1. Proceed to transect and collect 50 g samples of 1.)ground cover plants and low hanging leaves, ensuring that you obtain five different plant samples, and 2.) dead leaves and decaying ground cover. 2. Once back in laboratory, prepare a Berlese funnel by connecting a test tube filled with 25 ml ethanol to the bottom of a funnel. Place a mesh screen within the funnel and, carefully, place the second 50 gram sample within the funnel. Seal the connection between the test tube and funnel with parafilm and place under a heat lamp for later investigation. This will be used to collect invertebrates in a later lab. Picture of sample here.  3. From the first 50 gram sample, locate and identify the five plant samples. Findings reported in Table One below. 4. Observe moss, lily, and mold slides prepared for you using two different types of microscopes. 5. Dissect parts of a lily flower and seeds/beans provided. Classify as monocot or dicot.
Observations, Results, & Conclusion:
Seed samples- the only seed sample that was obtained was a gymnosperm cone.
Observing Fungi- using the dissecting microscope, you can clearly see the black bread mold, Rhizopus stolonifer, as well as its components, the mycelium and sporangia. Its sporangia are significant because the fungi's maturity can be determined by the color of the sporangia. Also, sporangia house spores, the fungi's haploid gametes. For black mold in the zygomycete group, these can be seen as black specks under the dissecting microscope.