User:Mark X. Ling/Notebook/Micb 323/2009/01/13

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Experiment 1 (Jan 13, 2009) Part 1 PCR: lab partner: Crystal Lee Big mix for six reactions (μL): buffer:30 dNTP:48 glycerol: 60 Formamide: 15 PCR done: 1: Primer set 1 +DNA 1 2: Primer set 1 +DNA 2 3: Primer set 2 +DNA 1 4: Primer set 2 +DNA 2 5: Primer set 1 +no DNA (control) Primer set 1 : otrA1 and otrA3 Primer set 2: otrA1 and otrA2 Part 2: PCR modification: modify KCl: tube no.1: 10μL(67.5μL water) no.2: 0μL (77.5μL water) no.3: 20μL (57.5μL water) no.4: 70μL (7.5μL water) rest: tris: 10μL, MgCl2:7.5μL, tween: 5μL modified Mgcl 2 (μL): 0 ; 7.5 ; 20 ; 60 done by Crystal Lee Part3: Viral Growth Curve: Partners: Crystal, Marla, Anna and temperature of water:35 oC Time of phage/host mix: 3:01 above time is according to the room clock; computer time=3:03 T0 = 3:08; T1=3:13; T2=3:18; T3=3:23; T4=3:28; T5=3:38; T6=3:48; T7=3:58; T8=4:08; T9=4:18 the incubation time is all according to the computer time. Post Lab comments PCR preparation went smoothly. Temperature of water was slightly lower than one specified in manual. overall the procedures in this lab was conducted according to the manual and went smoothly