User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/11/07

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Bead Protocol Edited

  • 30 mL of ethyl acetate + 500 uL of glutaraldehyde
  • Add your PVA-clay (approximately .5 g of PVA and .051-.055g NaMT) in a dropwise manner. Immediately start timing the reaction for 2 minutes when you do the first drop.
  • After 2 minutes, slowly add 7 mL of 0.2 M NaHCO3.
  • Once completed, transfer to a falcon tube and centrifuge for 1 minute at 3000 rpm, rotor 41.
  • When beads are separated from the organic phase, pipet out the organic phase and re-suspend the beads in distilled water.

PVA-clay-HCl stock

  • 1.0016g PVA and 0.1114g clay in 12 mL of HCl


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