User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2012/05/23

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Objective

Prepping samples to bring to NIST for dynamic light scattering measurements tomorrow.

Description

I made 2 solutions (11.8 mg of HAuCl4·H2O in 14 mL of water and 23.2 mg of BSA in 22mL of water). I then proceeded to use these solutions to prepare my samples for tomorrow.

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Data

Something strange happened in the notes section ... Here is the data: I made 2 solutions (11.8 mg of HAuCl4 in 14 mL of water and 23.2 mg of Bovine Serum Albumin in 22 mL of water) and used these solutions to make samples for preparing AuNPs.

Approx. Ratio Au/BSA Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL)
20 0.50 4.167 0.333
30 0.50 2.777 1.723
40 0.50 2.083 2.417
50 0.50 1.667 2.833
60 0.50 1.389 3.111
70 0.50 1.190 3.310
80 0.50 1.042 3.458
90 0.50 0.927 3.573
100 0.50 0.833 3.667
110 0.50 0.757 3.743
120 0.50 0.693 3.807
130 0.50 0.640 3.860
140 0.50 0.594 3.906
150 0.50 0.556 3.944
160 0.50 0.520 3.980
170 0.50 0.49 4.010
} These samples were placed in the oven at 80°C for 4 hours. After 3 hours, this is what the solutions look like
Here is the UVVis Data part 1
Here is the UVVis Data part 2

Notes

This area is for any observations or conclusions that you would like to note.


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Approx. Au/BSA ratio Volume Au added (mL) Volume BSA added (mL) Volume Water added (mL) Actual Ratio
20 0.50 4.167 0.333 18.8
30 0.50 2.777 1.723 28.3
40 0.50 2.083 2.417 37.7
50 0.50 1.667 2.833 47.1
60 0.50 1.389 3.111 56.5
70 0.50 1.190 3.310 66.0
80 0.50 1.042 3.458 75.3
90 0.50 0.927 3.573 84.7
100 0.50 0.833 3.667 94.2
110 0.50 0.757 3.743 103.7
120 0.50 0.693 3.807 113.2
130 0.50 0.640 3.860 122.6
140 0.50 0.594 3.906 132.1
150 0.50 0.556 3.944 141.2
160 0.50 0.520 3.980 150.9
170 0.50 0.490 4.010 160.2


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