- Synthesize Pepsin-AuNPs for reactivity tests tomorrow
- Determine the Au:Protein ratio required for fiber formation for pepsin and HRP
- Synthesis of HRP-AuNPs and Pepsin-AuNPs
- Into test tube that will contain a total of 5mL add
- Au solution such that the final concentration is 0.25mM
- Protein solution such that the final concentration reflects the following Au:Protein ratios
- Water up to 5mL
- 67.3mg in 100mL of water ---> 15.3 μM
- 54.4mg in 100mL of water ---> 15.7 μM
- Need 50mL of 2.5mM
- 49.7mg of HAuCl4·3H2O in 50mL water --> 2.52 mM
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