User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/10/03

Biomaterials Design Lab

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Objective Acquire fluorescence scans of myoglobin at pH 4 and pH 5 at 80C. pH 4 fluorescence 3 mL Sample 1 μM myoglobin Stock Concentration 160 μM Volume Added: 18.75 μL pH 4 Stock Concentration 1 mM HCl Volume Added: 300 μL Volume water added: 2681 μL UV-Vis of sample Definite unfolding of myoglobin as heme is misligated. Fluorescence scans Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements pH 5 Fluorescence 3 mL Sample 1 μM myoglobin Stock Concentration 160 μM Volume Added: 18.75 μL pH 5 Stock Concentration 1 mM HCl Volume Added: 30 μL Volume water added: 2961 μL UV-Vis of sample Fluorescence scans Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements The initial scan for the pH4 looks much different than the initial scan for the pH 5 Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.