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Making FPLC buffers
1L of 25mM Tris, 50mM NaCl pH8
3.029g Tris, 2.922g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter
1L of 25mM Tris, 1M NaCl pH8
3.029g Tris, 58.44g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter
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