User:Matt Hartings/Notebook/Photosynthesis/2013/02/12
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DNA GelMaddie ran several PCR runs last week to try to mutate K31C Asc Hb to K31C T109C Asc Hb. I made an agarose gel (1% - 0.25g agarose in 25mL TAE Buffer) The lanes in the gel are
(each lane consists of 5uL DNA and 1uL loading buffer). Results Lane 2 showed nothing. Lanes 3-6 were really streaky. Potentially many different products, or perhaps too concentrated. Should run again more dilute. Hb extractionLast week we expressed WT Asc Mn-Hb and Ni-Hb. I am going to extract the Mn-Hb today.
Nyousha is making buffers for FPLC: 1L of 10mM Tris 50mM NaCl pH 8.5, 1L of 10mM Tris 1M NaCl pH 8.5 The buffers were filtered after they were made. |