User:Matt Hartings/Notebook/Photosynthesis/2013/11/06

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Objective

Maddie ran Mb from tris and KCl in MeOH at 45C this morning and also just opd and h2o2 in MeOH at 5. Take kinetic data for o-phenylenediamine + H2O2 reaction in methanol (15).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. No Mb used
    2. OPD
      1. 2.2mg OPD in 2.2mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 15C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_15_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan


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