User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/10/27
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The objective of today was to run the Bradford Analysis of Protease Degradations for 1 nM Protinase K.
The protocol is identical to the one used on 9/29/15 except the fibers were spun down at 300 rpm for 10 minutes, not 1500 rpm for 1 minute.
The graph below shows the absorbance of the samples as function of the wavelength of incident light when corrected. The six samples were first corrected for by subtracting the absorbance of the superblank of buffer and Bradford reagents. Then the samples were corrected for again by subtracting the results of the first correction by the blanks for their corresponding time periods. Then, the isosbestic point at 535 nm from each samples was subtracted from all the wavelengths of that sample.
The graph below shows the peak for the absorbance at 600 nm. For the first 90 minutes the absorbance is realtively stable at around 0.04 with dips at 15 minutes and 60 minutes. It is unknown if these dips are due to peptide concentration or human error. The absorbance then increases after 60 minutes. It would be desired to take additional samples past two hours to see how the additional time would affect the data.