User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/02/24
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Today's objectives were to:
We used the same Rhodamine and chloroauric acid samples as last week for all samples described today. We made a new lysozyme stock that was 46.79µM.
Incubation of Samples Synthesized on February 23, 2016 in Rhodamine B
Because we had incubated the samples that we synthesized on February 17, 2016 in Rhodamine B for 24 hours, we decided to incubate the samples that we synthesized on February 23, 2016 for 3.5 hours. Then, we could try to determine whether incubating the samples in Rhodamine for longer periods of time helped incorporate more Rhodamine into the fibers.
The following table shows the amount of Rhomdaine B added to each sample.
We then covered the tops of the test tubes in parafilm and incubated the samples at room temperature for 3.5 hours.
First, we took fluorescence measurements of Rhodamine in varying concentrations of AuNP.
MICHAEL CAN YOU PUT THE CONCENTRATIONS/DILUTIONS OF AUNP AND RHODAMINE YOU USED FOR THE CALIBRATION CURVE HERE THANKS
Start 540 nm End 700 nm Ex: 520 nm
Synthesis of AuNP Fiber Samples
We made new AuNP fiber samples according to the following table. (Note that we did not add any Rhodamine. We are going to add the Rhodamine to the samples on March 1, 2016 after the samples have been incubated in the oven.)
Each of the samples had a total volume of 5mL and were made in glass test tubes. We sealed the test tubes by covering the top with aluminum foil and then taping the foil down with labeling tape so that no vapors could escape.
We then incubated the samples in the oven at 80°C for four hours.
MATT PUT YOUR PROTOCOL HERE PLZ THANKSALOT:)
Data, Observations, and Analysis
Calibration Curve for Fluorescence of Rhodamine B as a Function of AuNP Concentration
MICHAEL CAN YOU PUT THE CALIBRATION CURVE HERE WHEN YOU'RE DONE THAT WOULD BE SUPER DUPER COOL THX
Fluorescence Data for Supernatants of Samples
Since only measured the fluorescence of one Rhodamine standard, we could not make a calibration curve to determine the change in the concentration of Rhodamine in the supernatant based on our fluorescence measurements of the supernatants. Next week, we will measure Rhodamine standards so that we can determine the change in Rhodamine concentration.
The above image shows the raw fluorescence data for each sample we measured. The fluorescence is shown as a function of the wavelength of fluorescence (nm).
Images of Samples Synthesized on February 17, 2016 After 24 Hour Incubation in Rhodamine
This image was taken after the samples had been incubating in Rhodamine for 24 hours. From left to right, the samples are:
The concentration of Rhodamine added to the A_200uM samples was 1µM. The concentration of Rhodamine added to the A_100uM samples was 0.1µM. The concentration of Rhodamine added to the A_50uM samples was 0.01µM. No Rhodamine was added to the au_lys_after sample; this sample was a blank.
Images of Samples Synthesized on February 23, 2016 Before Addition in Rhodamine
Images of Samples Synthesized on February 23, 2016 After 3.5 Hour Incubation in Rhodamine
From left to right, the samples in this image are:
HPLC Data for Acetylsalicylic Acid
MATT ALSO PLZ PUT UR DATA HERE THAT WOULD BE COOL