User:Megan L. Channell/Notebook/Horseradish/2013/10/22

Biomaterials Design Lab

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Objective Synthesize Pepsin-AuNPs for reactivity tests tomorrow Determine the Au:Protein ratio required for fiber formation for pepsin and HRP Procedure Synthesis of HRP-AuNPs and Pepsin-AuNPs Into test tube that will contain a total of 5mL add Au solution such that the final concentration is 0.25mM Protein solution such that the final concentration reflects the following Au:Protein ratios 30 50 70 90 110 130 150 170 190 210 Water up to 5mL Source: Dr. Hartings Data Solutions Made: UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant Pepsin UV-Vis HRP UV-Vis Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.