User:Melissa Novy/Notebook/CHEM-571/2012/10/31

Objectives

• Make solutions of lysozyme and HAuCl₄ at mole ratios of Au:lysozyme of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

Au-Lysozyme Solutions

• A 10501 μM solution of HAuCl₄ in distilled H₂O, made on 2012/09/05 was obtained.
• 5 mL of 70 μM solution of lysozyme in distilled H₂O was made.
  • Add 0.005 g of lysozyme to 5 mL of distilled H₂O.

  (70×10-6 M lysozyme)(0.005 L)=3.5×10-7 mol lysozyme
  (3.5×10-7 mol)(14300 g/mol)=0.005005 g lysozyme

• Please refer to Dhea Patel's entry for calculations, actual molarity, and exact volumes of water, HAuCl₄, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
• The Au-lysozyme solutions were wrapped in foil to prevent photodegradation and placed in an oven for 4 hours at 80°C.