- Make 6 sets of 50-mL LB media.
- Make 500 mL agar solution.
- Autoclave LB media and agar solution.
- Make 20 agar plates.
- Filter and dry LMT-95Ag, made on 2013/01/29.
- Obtain 6 clean, dry 250-mL Erlenmeyer flasks.
- Place 1.25 g of LB medium, obtained from Teknova, into each flask.
- Fill each flask with 50 mL deionized H2O.
- Cap each flask with aluminum foil and swirl until the LB dissolves.
- Autoclave the flasks according to the AU Biomaterials Design Lab protocol.
Agar Solution and Agar Plates
- Obtain a clean, dry 1000-mL Erlenmeyer flask.
- Place 12.5 g of LB medium, obtained from Teknova, into the flask.
- Fill the flask with 500 mL deionized H2O.
- Swirl the flask to dissolve the LB, then add 7.5 g Trypticase Soy Agar, obtained from Aldrich.
- Cap the flask with aluminum foil and autoclave according to the AU Biomaterials Design Lab protocol.
- Obtain 20 sterile 10-cm Petri dishes.
- Fill each Petri dish with 25 mL of autoclaved agar solution.
Filter and Dry LMT-95Ag
- LMT-95Ag was covered with aluminum foil and left to stir for 24 h. It was then filtered with Whatman 41 filter paper and a clean, dry filter flask and funnel. It was washed with 10 mL of (50:50) H2O-EtOH.
- LMT-95Ag was then scraped from the filter paper with a spatula and placed in an aluminum dish loosely capped with foil in an 80°C oven to dry overnight.
- The filtrate was reserved for further testing for sodium.
- LMT-95Ag turned from opaque white in color to opaque dark gray. This indicates possible oxidation of Ag.
- The filtrate was clear and colorless, but became transparent red-gray in color after about 30 min. The filtrate was subsequently covered in foil.