User:Meng Xiao He/Notebook/fall08/2008/10/30

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PCR redo 7-11 should be: cbb3III 5 cbb3I 3 ccoIII 3 DuoII 5 DuoI 3 12??????? use columns 4, 6, 9, 11 on gradient mxfg, with dmso in 11 (.3ul) clonewelled Digest BsmBI, overnight at 55, heat killed at 80 for 25min: ccb3 (both) cco (both) Duo (both) Rx: 15 ul of each PCR clonewell frag 3.55ul buffer 3 2ul BsmBI