User:Meng Xiao He/Notebook/fall08/2008/11/30

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Gm/sucrose selection all colonies were sucrose insensitive all Duo colonies, some cbb colonies, and one cco colony was Gm sensitive seems like sucrose selection isn't really working? Gm- colonies picked for platinum colony pcr, with complex conditions (11-30) new filter Duo the 1 MR-1 colony that appeared on new cco filter plate was grown o/n in salt-free LB and streaked onto sucrose plate