User:Meng Xiao He/Notebook/fall08/2008/12/15

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PCR retry

  • retried PCR using 15ul volume and all 3 outer primer sets, 12-8 conditions
  • note that 24 lane means sets of 12 are staggered:

Image:2008-12-15_pcr_plate.jpg

However, it seems that the ones with bands are the only PCRs that remotely worked; the bands are actually the upper bands: Image:12-16pcr_redo.jpg

Will have to troubleshoot this PCR...

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