User:Michael S. Bible/Notebook/571/2014/08/27

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Colloid v. Fiber Formation

  • Creating a 125 μM stock solution of Lysozyme
    • 50 mL Lysozyme solution, (1.25x10-4 M)(0.050 L)(14300 g/mol)
      • 0.0894g Lysozyme in 50 mL Volumetric Flask
  • Creating a 2.512 mM stock solution of HAuCl4
    • 50 mL HAuCl4 solution, (2.5x10-3 M)(0.050 L)(340 g/mol)
      • 0.043 g HAuCl4 in 50 mL Volumetric Flask

Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein. Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.

Kinetics of AuNP Formation

Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). The test tubes were covered with foil and placed in an 80°C hot water bath. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.

HAuCl4 Lysozyme H2O
0.5 mL 1.0 mL 3.5 mL
0.5 mL 0.5 mL 1.0 mL
0.5 mL 0.33 mL 4.17 mL
0.5 mL 0.25 mL 4.25 mL
0.5 mL 0.20 mL 4.30 mL
0.5 mL 0.167 mL 4.33 mL

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