User:Michael S. Bible/Notebook/571/2014/08/27
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Colloid v. Fiber Formation
Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein. Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.
Kinetics of AuNP Formation
Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). The test tubes were covered with foil and placed in an 80°C hot water bath. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.