# User:Michael S. Bible/Notebook/581/2014/09/24

Biomaterials Design Lab Main project page
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## Creating PVA Films from Exchanged Clays

Today we made films using the cation exchange clays begun on September 10. One of the films was made using the tributylhexadecylphosphonium bromide that our group started on 9/10 and the other was made using (1-hexadecyl)triphenylphosphonium bromide that Eleni's group started synthesizing on 9/10.

Our cation exchanged clays were made as follows:

• PVA + tributylhexadecylphosphonium bromide exchanged clay
• Mass of PVA = 1.0025g
• Mass of tributylhexadecylphosphonium bromide = .1100g
• Volume of Water = 7.0 mL
• PVA + (1-hexadecyl)triphenylphosphonium bromide exchanged clay
• Mass of PVA = 0.9995g
• Mass of (1-hexadecyl)triphenylphosphonium bromide = 0.1103g
• Volume of water = 7.2 mL

## Soaking PVA Films and PVA+Clay Films in R6G

A new experiment (using the protocol from 8/29) was begun today using 1 μM, 10 μM, and 20μM R6G solutions instead of Malachite Green solutions.

#### Preparation of solutions

• The three concentrations of R6G were created from a stock solution of 159 μM R6G.
• 1μM R6G - 0.629 mL of stock into 100mL - prepared by Eleni
• 10μM R6G - 6.289 mL of stock into 100mL - prepared by Michael
• 20μM R6G - 12.578mL of stock into 100mL - prepared by James

#### Calculations

V1*C1 = V2C2

V1 = V2C2/C1

Vstock = (100 mL)(1μM)/159μM) = 0.629 mL

Vstock = (100 mL)(10μM)/159μM) = 6.289 mL

Vstock = (100 mL)(20μM)/159μM) = 12.578 mL

## Data

The following chart details the initial masses of each of the films begun today.