User:Michael S. Bible/Notebook/581/2014/11/14

Biomaterials Design Lab

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Objective Perform an experiment to determine the kinetics of the absorption of Malachite Green by Activated Carbon. Description Preparation of Malachite Green solution by James .0996 g of MG in 500 mL of water UV-Vis calculated concentration: 146 ppm 136.75 mL of 146 ppm in 1000 mL UV-Vis calculated concentration: 23.3 ppm Activated Carbon vs. 10mL 23.3 ppm Malachite Green 10 mL of 23.3 ppm MG are added to .01 g of activated carbon for a certain period of time. after the period of time has passed, the solution is filtered from the activated carbon using a vacuum filter. UV-Vis spectra are recorded to calculate the amount of MG absorbed in the given amount of time. Time intervals: every 15s for the first two minutes, followed by every 30 seconds for the next two minutes, followed by every minute up to 7 minutes Each time interval was done in a separate scintillation vial because the experiment proceeds too quickly to simply sample a single solution repeatedly. Data The graph that follows was created using previously-made calibration curves for the concentration of Malachite Green in solution using UV-Vis Spectrometry: Notes Add notes here.