User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/09/15

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Objective Nanoparticle fiber synthesis begun on September 2nd was unsuccessful. Only nanoparticle solutions were created, so today we will try to synthesize new samples of Au-NP Fibers. Protocol Stock solutions of HAuCl4 and Lysozyme were prepared by Dr. Hartings using the procedure found here. 5mL and 1 mL samples were created using the following procedure: Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 276 μL Volume of water: 640 μL 5 mL samples Volume of gold stock: 422 μL Volume of protein stock: 1,380 μL Volume of water: 3,198 μL The protocol above was taken from Dr. Hartings's Notebook. The solutions were covered by aluminum foil and placed in the oven at 80°C for 4 hours. The masses of 99 one mL eppendorf tubes were taken and the average mass and standard deviation were determined. Additionally, each group's UV-Vis data for the lysozyme calibration curve was combined. Results Only 10 of the 1 mL samples successfully produced AuNP fibers. None of the 5 mL samples were successful. The average mass of one eppendorf tube is 1.02514 g with a standard deviation of 0.00537 g. The graph above was created using the abosrbance values at λ = 280 nm in the UV-Vis spectra of lysozyme collected by the entire class. The extinction coefficient for lysozyme at λ = 280 nm, calculated from this data set, is ε = 3.32·104M-1cm-1.