User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/09/06
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To prepare a zein protein film.
ethanol solution: 90% v/v solution = 9mL ethanol, 1mL water
10% w/v zein = 0.1 g/mL, 1g zein in 10 mL
30% glycerol on zein weight basis = 0.3g glycerol
0.3g glycerol x 1 mL / 1.261g = 0.238 mL
The resulting film was more brittle than the others and came off in pieces rather than a large round film. The cause is mostly likely too little glycerol.