User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/11/08

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Objective

To purify the proteins expressed in cells frozen on 09/29/11.

Description

  1. Make solutions for tomorrow.
    • 1 L 50 mM Tris (pH 9.02)
    • 1 L 50 mM Tris + 1 M NaCl (pH 9.05)
    • 2 batches of 4 L 50 mM Tris (pH 9.00)
  2. Filter both 1 L solutions.
  3. Defrost cells from 09/28/11 in a water bath.
  4. Sonicate cells: 30 seconds on, 30 seconds off, 3x.
  5. Centrifuge at 17,000 rpm for 1.5 hr.

Data

Notes


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