User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/12/06

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Objective To repeat experiment from 11/30/11 to see if consistent results occur. Description Create solutions for ratio of 166 Au:BSA Using 17.2 μM BSA stock solution and 2.9 mM HAuCl4 stock solution, combine 0.5 mL gold solution and 0.51 mL BSA solution. Dilute with 5 mL of water. Repeat to make a second solution. Cover with tin foil. Create solutions for ratio of 70 Au: BSA Using the same stock solutions, combine 0.5 mL gold solution and 1.2 mL BSA solution. Dilute with 5 mL of water. Repeat to make a second solution. Cover with tin foil. Put all solutions into the oven at about 75°C. Leave one of each ratio concentration in for the duration of lab (about 3 hours). Take one of each ratio concentration out for 10 minutes every half hour for 3 hours. Data Au/BSA = 70, taken out of the oven every 30 minutes Au/BSA = 70, left in the oven Au/BSA = 166, taken out of the oven every 30 minutes Au/BSA = 166, left in the oven The following graph shows the UV/Vis data obtained that compares the four solutions where Au/BSA = 70 and 166, and the technique was either to leave the solution in the oven or to take it out every 30 minutes. This graph shows a zoom-in at 550nm where the difference between the nanoparticle formation is clear. The 70 ratio solution that stayed in the oven had the most success in AuNP synthesis. Notes