User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/03/06

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Au/BSA Mole Ratios Main project page
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Objective

Experimental section for solution effects of refolding buffer:

https://docs.google.com/document/d/1W0ynzs6K51j4vTJHovB_dpGgD1pen7H2dKGgEI1H0Mc/edit


  • To determine the effect of the mole ratio of Au/BSA on AuNP synthesis.

Description

Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 90 Au/BSA
    • 110 Au/BSA
    • 130 Au/BSA
    • 150 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3.5 hr.


Effect of Tris Buffer on Protein Fibers

  1. Centrifuge AuNP fibers + 50 mM tris solution made on 02/15/12.
  2. Take UV-vis absorption data.
  3. Compare data to that obtained on 02/15/12, 02/21/12, and 02/28/12.

Data

The following graph shows the data obtained over the past 3 weeks for the effect of tris on a fiber solution over time.

Notes

After working on the BSA calibration curve last week, we noticed that this data collected weekly has begun to show the BSA peak around 280 nm.


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