User:Mingjie Li/Notebook/Light switch/2008/11/13

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ML1-1 Optimize gene: 1. Arabidopsis PHYB(NT): Length:1866; Sequence: ATGGTGAGCGGAGTGGGAGGCAGCGGAGGAGGAAGGGGCGGCGGAAGGGG AGGAGAGGAGGAGCCTAGCAGCAGCCACACCCCTAACAACAGGAGGGGAGGAGAGCAGGC TCAGAGCAGCGGAACCAAGAGCCTGAGGCCTAGGAGCAACACCGAGAGCATGAGCAAGGC CATCCAGCAGTACACCGTGGACGCCAGGCTGCACGCCGTGTTCGAGCAGAGCGGCGAGAG CGGCAAGAGCTTCGACTACAGCCAGAGCCTGAAGACCACCACCTACGGCAGCAGCGTGCC TGAGCAGCAGATCACAGCCTACCTGAGCAGAATCCAGAGGGGCGGCTACATCCAGCCTTT CGGCTGCATGATCGCCGTGGACGAGAGCAGCTTCAGGATCATCGGCTACAGCGAGAACGC CAGGGAGATGCTGGGCATCATGCCTCAGAGCGTGCCTACCCTGGAGAAGCCTGAGATCCT GGCCATGGGCACCGACGTGAGGAGCCTGTTCACCAGCAGCAGCAGCATCCTGCTGGAGAG GGCCTTCGTGGCCAGGGAGATCACACTGCTCAACCCTGTGTGGATTCACAGCAAGAACAC CGGCAAGCCTTTCTACGCCATCCTGCACAGGATCGACGTGGGCGTGGTCATCGACCTGGA GCCTGCTAGGACCGAGGACCCTGCCCTGAGCATCGCTGGAGCTGTGCAGAGCCAGAAGCT GGCCGTGAGGGCTATCAGCCAGCTGCAGGCCCTGCCTGGCGGCGACATCAAGCTGCTGTG CGACACCGTGGTGGAGAGCGTGAGGGACCTGACCGGCTACGACAGAGTGATGGTGTACAA GTTCCACGAGGACGAGCACGGCGAGGTGGTGGCCGAGAGCAAGAGGGACGACCTGGAGCC TTACATCGGCCTGCACTACCCTGCCACCGACATCCCTCAGGCCAGCAGGTTCCTGTTCAA GCAGAACAGGGTGAGGATGATCGTGGACTGCAACGCCACCCCTGTGCTCGTGGTGCAGGA CGACAGGCTGACCCAGAGCATGTGCCTGGTGGGCAGCACCCTGAGGGCCCCTCACGGCTG CCACAGCCAGTACATGGCCAACATGGGCAGCATCGCCAGCCTGGCCATGGCCGTGATCAT CAACGGCAACGAGGACGACGGCAGCAACGTGGCCAGCGGCAGGAGCAGCATGAGGCTGTG GGGCCTGGTGGTGTGCCACCACACCAGCAGCAGGTGCATCCCTTTCCCTCTGAGATACGC CTGCGAGTTCCTGATGCAGGCCTTCGGCCTGCAGCTGAACATGGAGCTGCAGCTGGCCCT GCAGATGAGCGAGAAGAGGGTGCTGAGGACCCAGACCCTGCTGTGCGACATGCTGCTGAG GGACAGCCCTGCCGGCATCGTGACCCAGAGCCCTAGCATCATGGACCTGGTGAAGTGCGA CGGCGCCGCCTTCCTGTACCACGGCAAGTACTACCCTCTGGGCGTGGCCCCTAGCGAGGT GCAGATCAAGGACGTGGTGGAGTGGCTGCTGGCCAACCACGCCGACAGCACCGGCCTGAG CACCGACAGCCTGGGCGACGCTGGATACCCTGGAGCTGCCGCCCTGGGCGACGCCGTGTG CGGCATGGCCGTGGCCTACATCACAAAGAGGGACTTCCTGTTCTGGTTCAGGAGCCACAC CGCCAAGGAGATCAAGTGGGGCGGCGCCAAGCACCACCCTGAGGACAAGGACGACGGCCA GAGGATGCACCCTAGGAGCAGCTTCCAGGCCTTCCTGGAGGTGGTGAAGAGCAGGAGCCA GCCTTGGGAGACCGCCGAGATGGACGCCATCCACAGCCTGCAGCTGATCCTGAGGGACAG CTTCAAGGAGAGCTGA 2:Arabidopsis PIF3: Length:1575; Sequence: ATGCCTCTGTTCGAGCTGTTCAGGCTGACCAAGGCCAAGCTGGAGAGCGC CCAGGACAGGAACCCTAGCCCTCCTGTGGACGAGGTGGTGGAGCTGGTGTGGGAGAACGG CCAGATCAGCACCCAGAGCCAGAGCAGCAGGAGCAGGAACATCCCTCCTCCTCAGGCCAA CAGCAGCAGGGCCAGGGAGATCGGCAACGGCAGCAAGACCACCATGGTGGACGAGATCCC TATGAGCGTGCCTAGCCTGATGACCGGCCTGAGCCAGGACGACGACTTCGTGCCTTGGCT GAACCACCACCCTAGCCTGGACGGCTACTGCAGCGACTTCCTGAGGGACGTGAGCAGCCC TGTGACCGTGAACGAGCAGGAGAGCGACATGGCCGTGAACCAGACCGCCTTCCCTCTGTT CCAGAGGAGGAAGGACGGCAACGAGAGCGCCCCTGCCGCCAGCAGCAGCCAGTACAACGG CTTCCAGAGCCACAGCCTGTACGGCAGCGACAGGGCCAGGGACCTGCCTAGCCAGCAGAC CAACCCTGACAGGTTCACCCAGACCCAGGAGCCTCTGATCACAAGCAACAAGCCTAGCCT GGTGAACTTCAGCCACTTCCTGAGGCCTGCCACCTTCGCCAAGACCACCAACAACAACCT GCACGACACCAAGGAGAAGAGCCCTCAGAGCCCTCCTAACGTGTTCCAGACCAGGGTGCT GGGCGCCAAGGACAGCGAGGACAAGGTGCTGAACGAGAGCGTGGCCAGCGCCACCCCTAA GGACAACCAGAAGGCCTGCCTGATCAGCGAGGACAGCTGCAGGAAGGACCAGGAGAGCGA GAAGGCTGTGGTGTGCAGCAGCGTGGGCAGCGGCAACAGCCTGGACGGACCTAGCGAGAG CCCTAGCCTGAGCCTGAAGAGGAAGCACAGCAACATCCAGGACATCGACTGCCACAGCGA GGACGTGGAGGAGGAGAGCGGCGACGGAAGGAAGGAGGCTGGACCTAGCAGGACCGGCCT GGGCAGCAAGAGGAGCAGGAGCGCCGAGGTGCACAACCTGTCTGAGAGGAGGAGGAGGGA CAGGATCAACGAGAAGATGAGGGCTCTGCAGGAGCTGATCCCTAACTGCAACAAGGTGGA CAAGGCCAGCATGCTGGACGAGGCCATCGAGTACCTGAAGAGCCTGCAGCTGCAGGTGCA GATCATGAGCATGGCCAGCGGCTACTACCTGCCTCCTGCCGTGATGTTCCCTCCTGGAAT GGGACACTACCCTGCTGCTGCTGCTGCTATGGCTATGGGAATGGGAATGCCTTACGCTAT GGGCCTGCCTGACCTGAGCAGGGGAGGCAGCAGCGTGAACCACGGACCTCAGTTCCAGGT GAGCGGAATGCAGCAGCAGCCTGTGGCTATGGGCATCCCTAGGGTGAGCGGAGGAGGAAT CTTCGCTGGCAGCAGCACCATCGGAAACGGCAGCACCAGGGACCTGAGCGGCAGCAAGGA CCAGACCACCACCAACAACAACAGCAACCTGAAGCCTATCAAGAGGAAGCAGGGCAGCAG CGACCAGTTCTGCGGCAGCAGCTGA 3: Ho1: Length:723; Sequence: ATGAGCGTGAACCTGGCCAGCCAGCTGAGGGAGGGCACCAAGAAGAGCCA CAGCATGGCCGAGAACGTGGGCTTCGTGAAGTGCTTCCTGAAGGGCGTGGTGGAGAAGAA CAGCTACAGGAAGCTGGTGGGCAACCTGTACTTCGTGTACAGCGCCATGGAGGAGGAGAT GGCCAAGTTCAAGGACCACCCTATCCTGAGCCACATCTACTTCCCTGAGCTGAACAGGAA GCAGAGCCTGGAGCAGGACCTGCAGTTCTACTACGGCAGCAACTGGAGGCAGGAGGTGAA GATCAGCGCCGCTGGACAGGCCTACGTGGACAGGGTGAGGCAGGTGGCTGCTACCGCTCC TGAGCTGCTGGTGGCTCACAGCTACACCAGGTACCTGGGCGACCTGAGCGGAGGCCAGAT CCTGAAGAAGATCGCCCAGAACGCCATGAACCTGCACGACGGCGGCACCGCCTTCTACGA GTTCGCCGACATCGACGACGAGAAGGCCTTCAAGAACACCTACAGGCAGGCCATGAACGA CCTGCCTATCGACCAGGCCACCGCCGAGAGGATCGTGGACGAGGCCAACGACGCCTTCGC CATGAACATGAAGATGTTCAACGAGCTGGAGGGCAACCTGATCAAGGCCATCGGCATCAT GGTGTTCAACAGCCTGACCAGGAGGAGGAGCCAGGGCAGCACCGAAGTGGGCCTGGCCAC CAGCGAGGGCTGA 4.PcyA: Length:747; Sequence: ATGGCCGTGACCGACCTGAGCCTGACCAACAGCAGCCTGATGCCTACCCT GAACCCTATGATCCAGCAGCTGGCCCTGGCCATCGCCGCCAGCTGGCAGAGCCTGCCTCT GAAGCCATACCAGCTGCCTGAGGACCTGGGCTACGTGGAGGGCAGGCTGGAGGGCGAGAA GCTGGTCATCGAGAACAGGTGCTACCAGACCCCTCAGTTCAGGAAGATGCACCTGGAGCT GGCCAAAGTGGGCAAGGGCCTGGACATCCTGCACTGCGTGATGTTCCCTGAGCCTCTGTA CGGCCTGCCTCTGTTCGGATGCGACATCGTGGCTGGACCTGGAGGCGTGAGCGCCGCTAT CGCTGACCTGAGCCCTACCCAGAGCGACAGGCAGCTGCCTGCTGCCTACCAGAAGAGCCT GGCTGAGCTGGGACAGCCTGAGTTCGAGCAGCAGAGGGAGCTGCCTCCTTGGGGAGAGAT CTTCAGCGAGTACTGCCTGTTCATCAGGCCTAGCAACGTGACCGAGGAGGAGAGGTTCGT GCAGAGGGTGGTGGACTTCCTGCAGATCCACTGCCACCAGAGCATCGTGGCCGAGCCTCT GAGCGAGGCCCAGACCCTGGAGCACAGGCAGGGCCAGATCCACTACTGCCAGCAGCAGCA GAAGAACGACAAGACCAGGAGGGTGCTGGAGAAGGCCTTCGGCGAGGCCTGGGCCGAGAG GTACATGAGCCAGGTGCTGTTCGACGTGATCCAGTGA Order plasmid: pCL-CTIG Design primer for first sequencing: pML1-1F:gtaacaactccgccccatt Wrong！This sequence is repeated. pML1-1F': ttgacgtcaatgggtggagt pML1-1R:tccagctcgaccaggatg Design primer for second sequncing: pML1-1F': ttgacgtcaatgggtggagt pML1-1R2: ACGTCCTCGCCGTCTAAGT sequencing result GGGACACACGCGCAGACTGTCGACGGCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGCGAGGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGACATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACTCCGCCCCNTACGGCGCTCTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTTACCGATGCCCTTGGAATTGACGAGTACGGTGGGTAGGGGGCGCGAGGATCCCCCGGGCTGCAGGAATTCCTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGCTCGGTACCCGGGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCCCGAATTCGATCGTTGGTTTAAACCAACAACCGGTACCTCT-AGCGCTACCGGTCGCCAC