User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/28

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October 28, 2014

Primary Experiment #1 - KI Analysis

Because the titrations done last week demonstrated inconsistent results, the KI concentrations of each of the ten dialysis samples were re-analyzed using the self-made Iodide Ion Selective Electrode. The results are as follows:

Sample Potential (mV) ln[KI] [KI] (mM) Sample Potential (mV) ln[KI] [KI] (mM)
L+50 mM KI-253.73.01425925120.3739933450 mM KIN/AN/AN/A
L+25 mM KI-237.72.32499030710.2265809625 mM KI-240.12.42838064911.34050295
L+10 mM KI-215.81.381553443.98108119710 mM KI-210.81.1661568953.209633945
L+5 mM KI-199.90.6965924272.0069023765 mM KI-196.20.5371989831.711207024
L+2 mM KI-177.4-0.2726920260.7613272212 mM KI-173.7-0.4320854690.649153892


NOTE: No more 50 mM KI sample remained to test with I- ISE due to an error that occurred during the titration.

Primary Experiment #3 - Work-up

0.6 g/L Lysozyme vs CaCl2 using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozome
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2

Necessary Analyses:

Bradford

  • 5 lysozyme samples, 0.6 g/L lysozyme reference, and Bradford blank
  • 50 μL sample, 200 μL 1:4 Bradford, 750 μL 50 mM Tris/50 mM NaCl

Fluorescence

  • 5 lysozyme samples, 0.6 g/L lysozyme reference
  • 10 μL of sample in 990 μL of DI water

UV-Vis

  • 5 lysozyme samples, 0.6 g/L lysozyme reference
  • 10 μL of sample in 990 μL of DI water

NOTE: The 5 µM sample was re-run because the wavelength range was not correct the first time the sample(s) was/were run. A lysozyme stock reference sample and Bradford/Tris/NaCl blank were re-run for comparison as well.

CaCl2 ISE

Concentration Lysozyme Solution Potential (mV) Soak Solution Potential (mV)
50 mM88.687.8
5 mM61.363.5
0.5 mM30.039.0
50 µM10.516.3
5 µM8.926.0

Primary Experiment #2 - Set-up Take 2

30:1 colloid solution vs CaCl2 using MWCO 3500

NOTE: Because the results of the Bradford analysis of this dialysis (take 1) were ridiculous, for lack of a better word, this dialysis was re-run and re-analyzed.

Well 1 Well 2 Well 3 Well 4 Well 5
30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2



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