User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/11/04

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November 4, 2014

Primary Experiment #4

This dialysis leaked over the weekend.... Fail.

Primary Experiment #5 - Set-up

30:1 colloid solution vs KI using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid
2 mM KI 5 mM KI 10 mM KI 25 mM KI 50 mM KI

Enzyme Kinetics

Solution Preparation

    • 25 mL of 66 mM Potassium phosphate buffer, pH 6.24 was prepared in a beaker
    • Half of the buffer was placed in a Falcon tube, labeled & refrigerated overnight
    • 10 mL 0.01 wt% Micrococcus lysodeikticus Cell Suspension was prepared using the phosphate buffer
    • The cell suspension concentration was adjusted until the Absorbance at λ = 450 nm was between 0.6 & 0.7
    • The full protocol is here

See Paul's notebook for additional details.


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