User:Nader A. Khamis/Notebook/Experimental Biological Chemistry AU Fall 2011/2011/11/30

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Objective

  • Conduct synthesis using HAuCl4 to BSA molar ratios of 70 and 166 to determine effects of the two molar ratios on the formation of purple solution or fibers in a constant or changing temperature enviroment.

Description

  • A 17.7µM of BSA Stock Solution was created using 0.0117g BSA and 10 mL H2O.
  • Two solutions, A and B, containing a HAuCl4/BSA molar ratio of 70 were prepared in eppendorf tubes using the following procedure.
  1. Add 0.042 mL of HAuCl4 (2.9mM)
  2. Add 0.1 mL of BSA (17.7µM)
  3. Add 0.85 mL of water

Solution A was placed in the 80°C heatblock for 30 minutes, placed in room temperature for 10 minutes, and was placed back into the heat block for 30 minutes over a period of 2 hours.

Solution B was kept in the 80°C heatblock for 2 hours.

  • Two solutions, C and D, containing a HAuCl4/BSA molar ratio of 166 were prepared in eppendorf tubes using the following procedure.
  1. Add 0.1 mL of HAuCl4 (2.9mM)
  2. Add 0.1 mL of BSA (17.7µM)
  3. Add 0.8 mL of water

Solution C was kept in the 80°C heatblock for 2 hours.

Solution D was placed in the 80°C heatblock for 30 minutes, placed in room temperature for 10 minutes, and was placed back into the heat block for 30 minutes over a period of 2 hours.

Data

  • For Solutions A and B, both were transparent purple solutions.
  • For Solutions C and D, both contained purple protein fibers in a clear solution.

At a molar ratio of 70, both solutions A and B yielded transparent purple solutions despite changes in temperature. Similarly, At a molar ratio of 166, purple protein fibers appeared whether a solution was left in the heatblock or was removed every thirty minutes.

Image: Experimental_Biological_Chemistry_-_November_30th_2011.jpg‎ From top to bottom: A, B, C, and D.

Notes

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