User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/04/08

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Objectives

  • Centrifuge 2nd procedure Ag:BSA NPs
  • UVvis of Ag:BSA NPs from last time
  • Put dry-autoclaved scaffolds into bacteria
  • Make Au nanofibers and print scaffolds (12) for brute force

Sample Prep: Nanofibers for Brute Force

Image:2014_0211_myo_bsa_NPs.PNG

  • Solutions:
    • Au: 0.011g in 10mL to make 2.793mM solution
    • Myoglobin: 0.011g in 10mL to make 0.0621mM solution

Brute Force Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above method
  • Nanofibers dried onto PLA scaffolds
  • Scaffolds submerged in water
  • Autoclaved

Results: UV-vis

Image:2014 0408 abs AgBSA NPs.PNG

  • Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm

Sample Prep: Scaffolds in Bacteria

  • E. coli bacteria culture were prepared
  • Bacteria culture media was prepared and placed in 5 small test tubes, each tube for each type of scaffold treatment
  • E. coli were placed in test tubes
  • 3 Dry-autoclaved scaffolds submerged in each test tube of bacteria solution
  • Test tubes with cells and scaffolds were placed in shaker until next class



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