User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/04/27

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April 27th, 2010

Because the last trials of getting a double terminator (BBa_B0015)didn't work we decided getting the double terminator from a petri box grown by Jorge instead of the petri box we had been using.
I grew 4 colonies (1, 3, 4 and 8) from the box containing the plasmid pSB1AK3, in medium LB with 4μL of kanamycin 30; after 7h30 I extracted the plasmid in 4 different tubes (one per colony) with the High Pure Plasmid Isolation Kit.



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